FIG. 6.

Localization of Bni1-GFP and the Spitzenkörper. (A) A BNI1-GFP-expressing strain (BNI1/BNI1-GFP) was induced with serum and stained with FM4-64. GFP fluorescence and differential inference contrast (DIC) images were used in an overlay showing the colocalization of Bni1-GFP with the FM4-64-stained Spitzenkörper. (B) Simultaneous localization of Bni1-GFP to the hyphal tip and to a future septal site. Note that in the DIC image no septum is apparent. (C) Comparison of Spitzenkörper morphologies in the wild-type (wt) and bni1 strains using FM4-64. Images of FM4-64-stained germ tubes that were induced by serum at 37°C for 3 hours are shown. Bars, 5 μm.