TABLE 2.
Oligonucleotide primers used in this study
| Primer | Sequencea |
|---|---|
| 392 XFP | GAGTGCCATGCCCGAAGGTTATG |
| 599 U3 | GGAGTTGGATTAGATGATAAAGGTGATGG |
| 600 U2 | GTGTTACGAATCAATGGCACTACAGC |
| 601 H2 | CAACGAAATGGCCTCCCCTACCACAG |
| 602 H3 | GGACGAATTGAAGAAAGCTGGTGCAACCG |
| 797 S1-BNI1 | ATCACAAACATCTTTTCATCATCCACTACTATCACCAGTGCTAGTGCAGTTTCAATTAATTACCATATTCTTCAACCTCATCCTCCCTTACCTCCTCCCTgaagcttcgtacgctgcaggtc |
| 798 S2-BNI1 | CTTAACTTGTAAACTCACATGGTATATAATATGTAAATACAACTTTGTACAGATATAAAAATTAATAGCTATATTTCATTTAAATACATATACAAAAAAAAtctgatatcatcgatgaattcgag |
| 799 G1-BNI1 | CTCTCTGAGGGAGACAACGC |
| 800 G4-BNI1 | CAGGTTTCCAAATTGAATCGTCC |
| 801 G4-BNI1-MAL2p | GCACCCTTGTCGTATGGAC |
| 802 G1-BNI1-FP | CCGCCTGAAGGATGCTG |
| 804 S2-BNI1-MAL2p | GAGTTGAGTTCTGACTTGCTGATGATAATATACTAGCTGAGTCATTGGAAGACATGTCGGACACACTGTCGGTGTTGTGCTTGTCT TTATGTCGTCTCCTcattgtagttgattattagttaaaccac |
| 805 S1-BNR1 | GTTGTTTTTTTTTTTCAAACGCGACTTCTAAATCTCTAGCCAT ACCCGATCCAGAAACACTTGTTTTAAATTTTTTTGGTTACCACACACACAAAAATATTCgaagcttcgtacgctgcaggtc |
| 806 S2-BNR1 | AGAAAGTGAAAAAAAAGAAAAAAGAAAAAAAAAAAATAGTTGTTCTTTTTTAAGGAAGAGCATCACAAAATTTTTTAGACGTGTAT ATGCAGTATCGGTGTAGtctgatatcatcgatgaattcgag |
| 811 G1-BNR1 | GTAAGCACCGAGTCTTGTCGC |
| 812 G4-BNR1 | GGAAATTTCTACTCAACGAGCG |
| 1088 I1-BNI1 | GGAAATCAAGAACCAGAGCCTTG |
| 1089 I2-BNI1 | CTCTTGGCAAAGCCGGCAACAC |
| 1090 I1-BNR1 | GAGATAGATTCCAGGAACACGAG |
| 1091 I2-BNR1 | CACCAATGCCTTGACGACGTACAC |
| 1203 CaBNI1 | CGCGGATCCGCGGGCTCACCAACTAATGTCTCACC |
| 1204 CaBNI1 | TGCTCTAGAGCACGACTCTATTTATGATGACGAAGATGAAG |
| 1242 ADE2-down | GGTCGTATGATTGTTGAAGCAGCAC |
| 1243 ADE2-up | CCAGAGTTGTGAGGTCTTGGTGC |
| 1244 RAS1-down | GGAAAGACAAGTTAGTTATCAAGATGG |
| 1268 S1-BNI1-GFP | GTTCAAATAGATCTTGATGAAGTGGCTAAGAATAACAATAGTGAGggtgctggcgcaggtgcttc |
| 1269 S2-BNI1-GFP | CTCGAATTCATCGATGATATCAGAGGCCTGCTAAGGAGAAGCACTtttttttgtatatgtatttaaatg |
a
Uppercase sequences correspond to C. albicans genomic DNA. Lowercase sequences correspond to 3′-terminal annealing regions for the amplification of transformation cassettes. Bold letters indicate restriction sites used for cloning. All sequences are written from 5′ to 3′.