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Purification of the AEH from A. turbidans ATCC 9325
| Purification step | Total vol (ml) | Total protein (mg) | Total activitya (cexU) | Sp acta (cexU/mg) | Purification (fold) | Recovery (%) |
|---|---|---|---|---|---|---|
| Cell extract | 165 | 461 | 599 | 1.3 | 1 | 100 |
| CM-Sepharose | 32 | 31 | 477 | 15 | 12 | 80 |
| Hydrophobic interaction | 5.8 | 1.1 | 68 | 62 | 48 | 11 |
| Gel filtration | 2.0 | 0.033 | 22 | 667 | 513 | 3.7 |
Cephalexin synthesis activity.
