TABLE 3.

Purification of AEH from E. coli BL21(DE3)pLysS(pETAT)

Purification step	Total vol (ml)	Total protein (mg)	Total activity (cexU)	Sp acta (cexU/mg)	Purification (fold)	Recovery (%)
Cell extract	184	1174	2348	2	1	100
DEAE	195	60	2050	34	17	87
CM-Hapb	80	5.8	1109	191	96	47
Hydrophobic interaction	32	0.96	633	605	303	27
Gel filtration	6.6	0.18	153	859	430	7

^aCephalexin synthesis activity.

^bCM-Hap, ceramic hydroxyapatite.