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. 2005 Oct;4(10):1662–1676. doi: 10.1128/EC.4.10.1662-1676.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 3. — Southern blot analysis of the hap5Δ/hap5Δ mutant. (A) hap5Δ::URA3 and hap5Δ::HIS1 deletion alleles were generated as outlined in Materials and Methods. The complete coding sequence of CaHAP5 was removed and replaced with the indicated selectable markers. (B) Genomic DNAs were isolated from strains BWP17 (HAP5/HAP5), DMC104 (HAP5/hap5Δ::URA3), and DMC108 (hap5Δ::URA3/hap5Δ::HIS1), digested with PstI/HindIII, and analyzed by Southern blot hybridization using the probe indicated in panel A.