FIG. 9.

C. albicans CCAAT-binding factor represses the expression of COX5 and CYC1. (A) Northern blot analysis of total RNAs isolated from C. albicans DMC146 (HAP5/HAP5), DMC120 (HAP5/hap5Δ), DMC117 (hap5Δ/hap5Δ), and DMC126 (hap5Δ/hap5Δ ARG4::HAP5) grown at 30°C in YNB-glucose + AA (pH 5.6) or YNB + AA (pH 5.6), as indicated. The membrane was hybridized with radiolabeled probes specific for COX5 and CYC1 mRNAs, as indicated. 26S rRNA was used to normalize RNA loading, and the ethidium bromide-stained rRNA is shown at the bottom of panel A. (B) Morphology of cells at the time of RNA isolation. Cells with the indicated genotypes were taken at the time of RNA isolation, fixed with 10% formaldehyde, and photographed. The growth medium for the strains is indicated to the left of each row.