FIG. 2.

Bas1 binds promoter sites in vivo under repressing and derepressing conditions. Cells expressing Myc-tagged Bas1 were grown in SC medium containing adenine (+ade) or lacking adenine (−ade) and prepared for chromatin immunoprecipitation assays using anti-Myc antibodies. (A) PCR amplification of serially diluted input DNA using primers specific to the POL1 open reading frame, the ARN1 promoter, and the ADE2 gene promoter. (B) PCR amplification of immunoprecipitated DNA using primers specific to POL1, ADE5,7, and HIS4. (C) PCR amplification of immunoprecipitated DNA using primers specific to ADE1, ADE2, ADE4, ADE16, and ARN1. The intensity of PCR bands was quantified on a phosphorimager, and calculations were performed as described in Table 3. I, input DNA.