FIG. 4.

Bas1 binds to the ADE regulon promoter in a strain lacking Pho2. ChIP analysis of Bas1-Myc₁₃ binding to the ADE1, ADE2, ADE4, ADE5,7, and HIS4 promoters in strains that express the Pho2 protein (left) and in strains that lack Pho2 (right). Gene-specific primers were used to amplify the ADE, HIS4, and POL1 promoters. ChIP values and the ratio were calculated as described in Table 3. The ratio of Bas1 bound under derepressing to repression conditions is listed under each −ade lane. I, input DNA.