FIG. 5.
Pho2 binds to ADE gene promoters in vivo only under derepressing conditions. Strain JRY134 (BAS1 PHO2-HA3::KanMX6) was grown on SC medium containing adenine or lacking adenine and prepared for ChIP assays using anti-HA antibodies. Primers specific to the indicated loci were used as described in the legend to Fig. 2. ChIP values and the ratios were calculated as described in Table 3. The ratio of Bas1 bound under derepressing to repressing conditions is listed under each −ade lane. I, input DNA; ChIP, chromatin immunoprecipitation.