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Pho2 is not recruited to ADE5,7 in a strain lacking Bas1. Strains RR409 (BAS1 PHO2-HA3) and RR412 (bas1-Δ10 PHO2-HA3) were grown in SC medium containing and lacking adenine and prepared for ChIP assays using anti-HA antibodies. Primers specific to ADE5,7 and POL1 were used in the PCRs.
