TABLE 2.
Activator-dependent lacZ reporter expression in epitope-tagged strains
| Strain/plasmid | Relevant genotype | β-Galactoside sp act (U)a
|
Fold repressionb | |
|---|---|---|---|---|
| +ade | −ade | |||
| AY854/pR116 | BAS1 PHO2 | 8.0 | 29.0 | 3.6 |
| AY856/pR116 | bas1-2 PHO2 | 8.0 | 7.2 | 0.9 |
| RR376/pR116 | BAS1-myc13 PHO2 | 9.0 | 29.3 | 3.3 |
| AY854/pR133 | BAS1 PHO2 | 0.7 | 3.6 | 5.1 |
| AY858/pR133 | BAS1 pho2-2 | 0.2 | 0.3 | 1.5 |
| JRY134/pR133 | BAS1 PHO2-HA3 | 0.5 | 6.6 | 13.2 |
a
The specific activity of β-galactosidase is expressed as nmol of o-nitrophenyl-β-d-galactopyranoside hydrolyzed per min per mg of protein and was measured in triplicate.
b
Fold repression is calculated as the β-galactosidase activity from cells grown in medium lacking adenine (−ade) divided by the activity from cells grown in medium containing adenine (+ade).