TABLE 3.
Quantification of the relative amounts of chromatin-immunoprecipitated DNA under repressing and derepressing conditions
| Locus | Amt of ChIP DNA relative to that fora:
|
|||||
|---|---|---|---|---|---|---|
|
POL1
|
ARN1
|
|||||
| +ade | −ade | Ratiob | +ade | −ade | Ratio | |
| ADE5,7 | 2.8 | 6.1 | 2.2 | 1.5 | 3.1 | 2.0 |
| ADE1 | 2.6 | 7.1 | 2.7 | 1.2 | 2.9 | 2.4 |
| ADE2 | 3.1 | 7.9 | 2.5 | 1.0 | 2.2 | 2.2 |
| ADE4 | 3.0 | 7.0 | 2.3 | 1.3 | 2.9 | 2.2 |
| HIS4 | 2.6 | 4.5 | 1.7 | NDc | ND | ND |
a
The value in each +ade or −ade column is a normalized ratio and was calculated as [ChIP (ADE)/input (ADE)] divided by [ChIP (control)/input (control)]. These values are an average of triplicate measurements with a standard deviation of less than 20%.
b
The derepression ratio is the signal detected in −ade medium divided by that in +ade.
c
ND, not determined.