TABLE 4.
Chromatin immunoprecipitation assays performed on plasmid and chromosomal DNA templates
| Plasmidd | Chromosome ChIPa
|
Plasmid ChIPb
|
β-Galactosidasec
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| −ade | +ade | Ratioe | −ade | +ade | Ratioe | −ade | +ade | Ratioe | |
| None | 3.0 | 1.6 | 1.9 | ||||||
| Wild type | 1.2 | 0.5 | 2.2 | 0.7 | 0.3 | 2.3 | 290 | 15 | 19 |
| Proximal site | 1.2 | 0.6 | 2.0 | 0.7 | 0.4 | 1.8 | 64 | 11 | 5.8 |
| Distal site | 0.9 | 0.5 | 1.9 | 0.3 | 0.5 | 0.6 | 5.4 | 6.1 | 0.9 |
a
ChIP assays were performed using the primer pair (IO-401 and IO-402) specific to the chromosomal locus of ADE5,7 and relative to POL1, as in Table 3.
b
ChIP assays were performed using the primer pair (IO-403 and IO-404) specific to the plasmid-borne locus of ADE5,7 and relative to POL1, as in Table 3.
c
β-Galactosidase activities reported previously (31) are reprinted here for ease of comparison.
d
Plasmids used were pR136, carrying the wild-type ADE5,7 promoter, pR139, carrying the proximal site (distal site mutation), and pR152, carrying the distal site (proximal site mutation) (31).
e
Ratio is calculated as the −ade value divided by the +ade value.