FIG. 4.
DNA synthesis in G1 cells during recovery and development. DNA synthesis was monitored by BrdU incorporation as described for Fig. 3. (A) BrdU incorporation in different samples. Lanes: 1, spores allowed to germinate and recover in growth medium for 16 h with BrdU; 2, cells allowed to recover in growth medium for 16 h with BrdU and then allowed to develop for 30 h without BrdU; 3, recovered cells with BrdU added upon initiation of development (0 to 30 h); 4, spores produced by cells treated as for sample 3; 5, recovered cells developing from 0 to 6 h of with BrdU and 6 to 30 h without BrdU; 6, vegetative cells developing with BrdU for 30 h; 7, vegetative cells grown with BrdU for 8 h (one cell doubling). rDNA, rDNA palindrome. (B) MtDNA detected by Southern blot hybridization.