TABLE 3.
mtDNA synthesis during germination and developmenta
| Expt | Cell category and treatment prior to harvesting (treatment duration) | mtDNA synthesis (% of mtDNA) |
|---|---|---|
| 1 | Vegetative cells grown in medium + BrdU for one cell doubling | 100 |
| 2 | Spores germinated and recovered in medium + BrdU (16 h) | 36 ± 9.8 |
| 3 | Spores germinated and recovered in medium + BrdU (16 h) and developed − BrdU (30 h) | 18 ± 10 |
| 4 | Vegetative cells grown in medium − BrdU (16 h) and developed + BrdU (30 h) | 70 ± 8.5 |
| 5 | Spores germinated and recovered in medium − BrdU (16 h) and developed + BrdU (30 h) | 26 ± 9.5 |
| 6 | Spores germinated and recovered in medium − BrdU (16 h) and developed + BrdU (30 h); spores only were harvested | 34 ± 7.0 |
| 7 | Vegetative cells grown in medium − BrdU (16 h) and developed − BrdU (0-6 h), then + BrdU (6-30 h) | 23 ± 6.0 |
| 8 | Spores germinated and recovered in medium − BrdU (16 h) and developed − BrdU (0-6 h), then + BrdU (6-30 h) | 13 ± 7.0 |
a
The mtDNA synthesis of developing cells was quantified from BrdU incorporation measured in experiments as described for Fig. 4. The BrdU incorporation observed in each band is shown relative to that observed during one cell cycle of G2 cells growing in liquid medium and normalized to the total amount of mtDNA in that band. Except for the experiment whose results are shown in row 6, all cells were harvested for processing.