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. 2005 Oct;71(10):6418–6422. doi: 10.1128/AEM.71.10.6418-6422.2005

FIG. 1.

FIG. 1.

Results of groEL PCRs and HindIII digestion. Lane MW contained molecular weight standards. Lane 1 contained amplicon obtained after the first PCR round starting with DNA extracted from the buffy coat of a symptomatic dog. Lanes 2, 4, 6, and 8 show groEL heminested PCR results obtained using internal primer EPhGroR, specific for A. phagocytophilum, with DNA extracted from the reference strain of A. phagocytophilum and from samples Stechi, Sogno, and Perla, respectively. To the right of each PCR result, the corresponding HindIII digestion result is shown (lanes 3, 5, 7, and 9). Lanes 10 and 12 show groEL heminested PCR results obtained using internal primer EPlGroR, specific for A. platys, with DNA extracted from the control strain of A. platys and from sample Lara, respectively. To the right of each PCR result, the corresponding HindIII digestion result is shown (lanes 11 and 13). Lane 14 shows HindIII digestion of the amplicon obtained after the first PCR (lane 1) and identification of the sample as A. platys.