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. 2002 Jan;68(1):166–172. doi: 10.1128/AEM.68.1.166-172.2002

TABLE 1.

Stoichiometry of nitrogen and carbon during RDX degradation calculated based on percentage of reacted RDX using total theoretical numbers on N and C atoms

Element (total no. of atoms) NO2 N2O NH3a CO2 MW 119 % Recovered
Carbon (3) 30.0b (0.8)e 64.0c 94.0d
Nitrogen (6) 30.0 (1.7)e 1.5 (8.5)e 10.0 48.0f 89.5
a

No ammonium salt was added to the resting-cell suspension.

b

Based on 14CO2 measured using [U-14C]-RDX.

c

Based on HPLC/radioactivity measurement using [U-14C]-RDX.

d

Total of the measured values was 97.6%, distributed as follows: 30% was CO2 and 67.6% was in the liquid phase, leaving 3% unidentified.

e

Value in parentheses represents relative standard deviation of a triplicate.

f

Calculated based on the empirical formula C2H5N3O3 found by LC/MS (ES-) and high-resolution MS.