. 2005 Oct;71(10):5728–5734. doi: 10.1128/AEM.71.10.5728-5734.2005

TABLE 2.

Kinetic parameters of the parent and evolved PTDH variants in 50 mM MOPS (pH 7.25) at 25°C^a

Generation	Variant	k_cat (min⁻¹)	K_m (μM, NAD)	k_cat/K_m_,NAD (μM⁻¹ min⁻¹)	K_m (μM, Pt-H)
0	Parent	262	66	4.0	57
1	1-1A9	285	66	4.3	48
	1-8D6	244	70	3.5	78
	1-11A6	278	64	4.3	58
	1-23C7	238	60	4.0	45
	1-25E8	262	75	3.5	99
	4x	218	74	2.9	144
	5x	170	46	3.7	75
2	2-2C10	171	65	2.6	155
	2-9B6	198	52	3.8	92
	2-17C2	174	56	3.1	120
	7x	174	66	2.6	96
3	3-34G8	192	57	3.4	169
	3-57E11	210	63	3.3	105
	3-89D3	216	75	2.9	123
	3-110G7	216	71	3.0	156
	3-135H2	213	50	4.3	135
	12x	195	40	4.9	46

Average of all statistically relevant data (standard deviation <10%).