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Time courses of cycloamylose production by the wild-type amylomaltase (A) and the Y54F (B), Y54G (C), Y54K (D), Y54P (E), and Y54W (F) mutated enzymes. Squares, cycloamylose (CA) yield; diamonds, reducing power in the reaction solution. The reaction conditions and the method used to determine the amount of cycloamyloses are described in Materials and Methods. The reducing power when all of the amylose is broken down to glucose is defined as 100%.
