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Correlation between the ratio of hydrolytic activity to cyclization activity and the yield of cycloamylose (A) or the reducing power in the reaction solution (B). Squares, wild-type enzyme (WT); diamonds, Y54G mutated enzyme. The correlation coefficients (R2) for the ratio and the yield of cycloamylose (CA) and for the ratio and the reducing power were 0.94 and 0.96, respectively.
