FIG. 1.

Flow cytometry of dormant and germinated spores. Spores of PS533 (wild-type) either dormant (A), treated for 6 min with a pressure of 150 MPa (B), or killed 98% with H₂O₂ and treated for 7 min with a pressure of 150 MPa (C) were stained with Syto 16 and analyzed by flow cytometry as described in Materials and Methods. Arrows labeled days and g denote dormant and germinated spores, respectively. The fluorescence of dormant spores killed 98% with H₂O₂ was identical to that of dormant untreated spores, the fluorescence of untreated spores germinated for 30 min with l-alanine was identical to that of pressure-germinated spores, and the fluorescence of spores killed 97 and 98% with CuOOH and tBHP, respectively, and then treated for 7 min with a pressure of 150 MPa was the same as that of pressure-germinated H₂O₂-killed spores.