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Effect of spore DPA content on pressure germination. Spores of strains PS533 (wild type) or FB122 (sleB spoVF) prepared with or without DPA were treated with 150 MPa of pressure, and germination was assessed by flow cytometry as described in Materials and Methods. Symbols: ○, PS533 spores; •, FB122 spores prepared without DPA containing <3% of wild-type spore DPA levels; ▴, FB122 spores prepared with DPA and containing 77% of wild-type spore DPA levels.
