FIG. 4.

Effects of changes in unsaturated fatty acid composition and sporulation temperature on pressure germination. Spores of different strains were prepared at various temperatures and treated with 150 MPa of pressure, and germination was assessed by flow cytometry as described in Materials and Methods. (A) Spores prepared at 27°C (○, □, and ▾) and spores prepared at 40°C (•, ▪, and ▴). Circles, PS533 (wild type); squares, PS3624 (↑Des); triangles, PS3628 (Des). (B) PS533 spores prepared at 23°C (○), 30°C (•), 37°C (□), and 44°C (▪).