FIG. 5.

Pressure germination of spores killed by oxidizing agents. Spores of strain PS533 (wild type), either untreated or killed with H₂O₂ or tBHP, were exposed to a pressure of 150 MPa, and spore germination was assessed by flow cytometry as described in Materials and Methods. Symbols: ○, untreated spores; •, spores killed 96% with H₂O₂; ▴, spores killed 98% with tBHP. Spores killed 97% with CuOOH and exposed to a pressure of 150 MPa germinated at the same rate as the tBHP-treated spores.