TABLE 4.
Effects of inhibitors on pressure germination of sporesa
| Inhibitor (concn)b | Germination rate (% of maximum) |
|---|---|
| None | 100c |
| Amiloride (1 mM) | 102 |
| o-Chlorocresol (2 mM) | 67 |
| Ethanol (5%) | 97 |
| HgCl2 (2 mM) | 6 |
| Octanol (0.006%) | 104 |
a
Spores of strain FB87 (gerB gerK) were treated with a pressure of 150 MPa, and spore germination was assessed by flow cytometry as described in Materials and Methods.
b
These concentrations of inhibitors gave >85% (amiloride), >90% (HgCl2), and >95% (o-chlorocresol, ethanol, and octanol) inhibition of the rate of spore germination with l-alanine (9).
c
This value was set at 100%.