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Synechocystis culture supernatants. Culture supernatants shown above were collected by 25 min of centrifugation at 4,000 × g after 10 days of incubation at 30°C. The culture supernatant of Synechocystis sp. strain PCC 6803(pMON36547) incubated in BG110NO3− medium exhibits a characteristic brown color that is typical for bacteria excreting HGA (7).
