TABLE 3.

S. meliloti genes significantly repressed under iron-limiting conditions and in the rirA mutant^a

Open reading frame	Gene and/or description	Expression ratiob
		VMM	TY medium	ΔrirA
SMa0249	Conserved hypothetical protein	−1.12	−1.06	−0.07
SMa0252	Conserved hypothetical protein	−2.32	−1.09	−0.40
SMa1289	Hypothetical protein	−1.68	−1.53	−0.10
SMa2032	Putative nonheme chloroperoxidase	−1.70	−1.92	−1.11
SMb20293	Hypothetical protein	−1.47	−1.21	−0.03
SMb20397	Putative oxidoreductase protein	−2.85	−1.69	−0.47
SMb20649	nadE1, putative NH3-dependent NAD+ synthetase protein	−1.09	−1.62	−0.02
SMb20650	Putative long-chain fatty acid coenzyme A ligase protein	−1.18	−1.71	−0.02
SMb20651	Hypothetical protein	−2.30	−1.96	0.10
SMb20764	phnL, putative phosphonate uptake ABC transporter ATP binding protein	−2.76	−2.31	−0.29
SMb20986	narB, putative nitrate reductase large-subunit protein	−1.61	−1.39	0.39
SMb21170	Putative guanylate kinase	−1.08	−1.41	−0.25
SMb21174	phoT, phosphate uptake ABC transporter permease protein	−1.07	−1.75	−0.11
SMb21175	phoE, phosphate uptake ABC transporter permease protein	−1.70	−1.90	−0.31
SMb21177	phoC, phosphate uptake ABC transporter ATP binding protein	−1.36	−1.31	−0.15
SMc00158	Hypothetical protein	−1.41	−2.25	−0.22
SMc00159	Hypothetical protein	−1.70	−2.12	0.12
SMc00283	Putative transcription regulator protein	−3.82	−3.05	−0.64
SMc00335	rpsA, 30S ribosomal protein s1	−0.44	−1.14	−1.05
SMc00565	rplI, probable 50S ribosomal protein 19	−0.63	−1.12	−1.05
SMc00578	Hypothetical protein	−0.47	−2.05	−1.20
SMc00765	mcpZ, probable methyl-accepting chemotaxis protein	−3.68	−3.29	−0.23
SMc00887	Hypothetical protein	−3.35	−3.43	−0.50
SMc00888	Hypothetical protein	−3.08	−3.01	−0.08
SMc00975	mcpU, probable chemoreceptor (methyl-accepting chemotaxis) transmembrane protein	−4.11	−4.14	−0.81
SMc00986	Hypothetical protein	−1.83	−1.79	−0.45
SMc00998	Conserved hypothetical signal peptide protein	−1.63	−1.19	0.08
SMc01104	mcpX, probable chemoreceptor (methyl-accepting chemotaxis) transmembrane protein	−2.98	−3.67	−0.36
SMc01160	Putative transcriptional regulator protein	−2.51	−0.47	−1.17
SMc01319	rplJ, probable 50S ribosomal protein	−1.33	−1.10	−1.51
SMc01326	tufB, probable elongation factor Tu protein	−1.04	−0.42	−1.25
SMc01469	mcpW, probable methyl-accepting chemotaxis transmembrane protein	−4.38	−3.41	−0.63
SMc01842	Putative methyltransferase transcription regulator protein	−0.49	−2.05	−1.00
SMc01847	Putative methyltransferase protein	−1.40	−1.25	−0.92
SMc02392	Hypothetical protein	−1.39	−1.81	−0.76
SMc02634	Hypothetical transmembrane protein	−2.60	−2.12	−0.91
SMc03004	mcpE, putative chemoreceptor (methyl-accepting chemotaxis protein)	−1.36	−1.44	−0.57
SMc03005	Conserved hypothetical protein	−1.21	−1.85	−0.30
SMc03007	cheA, chemotaxis protein (sensory transduction histidine kinase)	−2.80	−3.04	−0.57
SMc03008	cheW1, chemotaxis protein	−2.04	−2.29	−0.37
SMc03009	cheR, chemotaxis protein methyltransferase	−4.21	−3.68	−0.43
SMc03012	cheD, chemotaxis protein	−2.18	−2.00	−0.63
SMc03013	Conserved hypothetical protein	−2.07	−2.03	−0.52
SMc03017	Conserved hypothetical protein	−1.61	−1.80	−0.84
SMc03021	fliM, flagellar motor switch transmembrane protein	−2.41	−2.83	−0.71
SMc03022	motA, chemotaxis (motility protein A)	−2.66	−2.81	−0.80
SMc03023	Conserved hypothetical protein	−2.42	−2.69	−0.76
SMc03024	flgF, flagellar basal body rod protein	−3.14	−3.36	−1.00
SMc03027	flgB, flagellar basal body rod protein	−3.25	−3.50	−0.84
SMc03028	flgC, flagellar basal body rod protein	−1.35	−1.74	−0.59
SMc03029	fliE, flagellar hook basal body complex protein	−3.20	−2.72	−0.79
SMc03030	flgG, flagellar basal body rod protein	−3.35	−3.30	−0.89
SMc03031	flgA, flagellar precursor transmembrane protein	−1.16	−1.88	−0.69
SMc03034	flgH, flagellar 1 ring protein precursor	−1.62	−1.97	−0.56
SMc03035	fliL, flagellar transmembrane protein	−2.66	−3.11	−1.19
SMc03037	flaA, flagellin protein	−3.95	−4.37	−0.11
SMc03038	flaB, flagellin protein	−3.97	−3.76	−0.42
SMc03040	flaC, flagellin protein	−2.28	−2.36	−0.45
SMc03042	motB, chemotaxis protein	−1.84	−2.00	−0.83
SMc03043	motC, chemotaxis protein precursor	−1.92	−2.49	−0.96
SMc03044	motD, chemotaxis protein	−2.97	−3.04	−0.82
SMc03045	Hypothetical transmembrane protein		−4.50	−1.41
SMc03047	flgE, flagellar hook protein	−1.66	−1.61	−0.50
SMc03048	flgK, putative flagellar hook-associated protein	−2.50	−1.90	−0.81
SMc03049	flgL, putative flagellar hook-associated protein	−3.14	−2.25	−0.85
SMc03051	flbT, putative flagellin synthesis repressor protein	−2.21	−1.90	−0.68
SMc03052	flgD, putative basal body rod modification protein	−1.94	−1.71	−0.64
SMc03057	Conserved hypothetical transmembrane protein	−1.57	−2.56	−0.74
SMc03071	Hypothetical protein	−1.68	−2.06	−0.56
SMc03072	Conserved hypothetical protein	−3.35	−2.48	−0.62
SMc03242	Probable GTP binding protein	−0.21	−1.10	−1.61
SMc03857	ffh, probable signal recognition particle protein	−0.84	−1.16	−1.64
SMc04007	Conserved hypothetical protein	−1.18	−0.86	−1.19
SMc04009	Conserved hypothetical protein	−0.85	−1.03	−1.14
SMc04059	Hypothetical protein	−2.86	−3.23	−0.66
SMc04300	afuC, probable iron ABC transporter ATPase	−0.75	−1.40	−1.39
SMc04317	afuA, probable iron ABC transporter periplasmic binding protein	−1.52	−1.55	−0.90

^aGenes were considered differentially expressed when according to t-statistics the P value was <0.05 with a repression level of >two fold. Only genes differentially expressed during at least two sets of microarray experiments were considered.

^bThe expression ratios are expressed as log₂ values.