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. 2005 Oct;71(10):5787–5793. doi: 10.1128/AEM.71.10.5787-5793.2005

FIG. 3.

FIG. 3.

Recombinant Cel5A expressed in E. coli and its CMCase activity. Proteins in gels containing 0.1% CMC were stained with Coomassie brilliant blue (lanes 1 to 3), and CMCase in gels containing 0.1% CMC was detected by Congo red staining (lane 4). Whole-cell lysates of both E. coli BL21(DE3) (control) (lane 1) and E. coli BL21(DE3) harboring plasmid pKY12 (lane 2) were included. The rCel5A expressed was partially purified with ProBond resin (lanes 3 and 4). The arrow indicates the position of the approximately 127-kDa band. The locations of molecular mass markers are indicated on the left.