TABLE 1.
DT-DGGE image analysis of bands showing substantial shifts in relative band intensity as a result of different incubation conditions
| Band | Closest BLAST relative (% homology) | % Intensitya (% change in RBI unitsb) under the following incubation conditions:
|
||||
|---|---|---|---|---|---|---|
| 100 μM H2S + NO3− | 1,000 μM H2S + NO3− | 5,000 μM H2S + NO3− | 1,000 μM S2O3 + NO3− | 1,000 μM S2O3 + O2 | ||
| Increased relative intensity following anoxic sulfidic incubations | ||||||
| FBR04D | Roseobacter sp. strain LA7 (98) | 61 (57) | 186 (261) | NM | 90 (67) | 100 (0) |
| FBR04C | Loktanella salsilacus (94) | 164 (191) | 296 (272) | NM | 98 (80) | 27 (−15) |
| DB04U | Rhodobium orientis (93) | NM | 76 (116) | 72 (100) | 64 (105) | 0 (ND) |
| FBR03B | Rhodobacter litoralis (99) | 32 (33) | NM | 102 (244) | NM | NM |
| Paracoccus denitrificans (97) | ||||||
| DB04R | Thauera aromatica (95) | NM | 28 (19) | 72 (300) | 40 (0) | 0 (ND) |
| DB04I | Thauera aromatica (99) | NM | 63 (−60) | 125 (58) | 71 (−37) | 36 (−79) |
| DB03B | Thauera aromatica (98) | 0 (ND) | NM | 50 (1058) | NM | NM |
| FBR03D | Thauera aromatica (98) | 12 (75) | NM | 34 (1400) | NM | NM |
| FBR03C | Hydrogenophaga taeniospiralis (98) | 21 (−6) | NM | 105 (153) | NM | NM |
| DB03D | Dethiosulfovibrio acidaminovorans (98) | 5 (100) | NM | 37 (1433) | NM | NM |
| Increased relative intensity following oxic sulfidic incubations | ||||||
| DB04H | Endosymbiont of scaly snail (95) | NM | 0 (ND) | 0 (ND) | 0 (ND) | 112 (#) |
| FBR04P | Sulfur-oxidizing bacterium ODIII6 (94) | 92 (64) | 126 (111) | NM | 70 (86) | 198 (418) |
| DB04G | Thiomicrospira sp. strain JB-A1 (92) | NM | 0 (ND) | 0 (ND) | 0 (ND) | 113 (#) |
| FBR04G | Thiomicrospira sp. strain JB-A1 (92) | 0 (ND) | 0 (ND) | NM | 0 (ND) | 241 (#) |
| DB04S | Thiomicrospira crunogena (96) | NM | 0 (ND) | 0 (ND) | 2 (#) | 25 (#) |
| Decreased relative intensity following sulfidic incubations | ||||||
| FBR04B | Nitrosomonas sp. strain Is343 (99) | 220 (−46) | 0 (ND) | NM | 241 (−43) | 281 (−51) |
| FBR04F | Uncultured Nitrosomonas strain AZP2-9 (99) | 115 (130) | 0 (ND) | NM | 165 (1.5) | 211 (4.4) |
| DB04A | Deep-branching bacterium from aromatic contaminated groundwater (80) | NM | 63 (−8.9) | 0 (ND) | 54 (5.0) | 100 (13) |
| DB04L | Planctomyces sp. strain LA4-B37N (85) | NM | 84 (−4.0) | 37 (−73) | 64 (−7.9) | 78 (−34) |
a
Relative to reference bands. NM, not measured.
b
Relative to the original untreated biofilter samples. ND, not detected; #, band not detected in the original sample.