TABLE 1.
Qualitative analysis of GAs and related metabolites in F. fujikuroi wild-type strain m567, F. proliferatum strains D02945 and D00502, and transformants of both F. proliferatum strains after complementation of the blocks in the first three enzymes of the pathwaya
| Strain | Products
|
|
|---|---|---|
| Culture filtrate | Mycelial extract | |
| m567 (MP-C) | GA3; GA13; fujenoic acid; GA3 isolactone; 7β,18-dihydroxykaurenolide; GA16; gibberellenic acid; GA7; GA25; GA1; GA9; GA4 | ent-kaurenoic acid; fujenoic acid; GA25; GA9; ent-kaurene; ent-kaurenal; ent-kaurenol |
| D02945 (MP-D) | ent-kaurenoic acid (trace) | |
| KT24 (D02945) | GA3; GA1; GA4; GA3 isolactone; fujenoic acid; ent-kaurenoic acid; GA13 | ent-kaurenoic acid; ent-kaurenal; ent-kaurenol; ent-kaurene |
| D00502 (MP-D) | GA1 (trace) | ent-kaurenoic acid (trace) |
| KT11 (D00502) | GA1; GA13; fujenoic acid | ent-kaurenoic acid; ent-kaurene; ent-kaurenal (trace) |
a
See the text for details. Strains were cultivated for 10 days in 20% ICI. Substances are listed in decreasing order of amounts. Experiments were repeated with similar results.