FIG. 2.

Time course of the irreversible loss of BMO activity during the consumption of 1,2-cis DCE (▵), 1,2-trans DCE (□), and 1,1-DCE (○) by butane-grown “P. butanovora.” Incubations were performed as described in the legend to Fig. 1, except that 50 μM DCE was used to ensure that the substrate was not limiting. Cells that did not receive DCEs served as positive controls (•). The data points are the means of three replicates, and the error bars represent standard deviations.