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Continuous culture of glutamate-producing C. glutamicum 2262 (A and B) and its non-glutamate-producing mutant 2262NP (C and D) at 39°C and a dilution rate of 0.05 h−1. (A and C) Optical densities at 570 nm (▴); (B and D) intracellular (×) and extracellular (⧫) glutamate concentrations. Arrows indicate the time when both the continuous supplementation of medium and the temperature increase from 33 to 39°C were imposed. The pH was maintained at 7.
