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. 2005 Oct;71(10):5920–5928. doi: 10.1128/AEM.71.10.5920-5928.2005

FIG. 4.

FIG. 4.

Transcriptional organization of the NCgl2816-lldD locus in C. glutamicum analyzed by RT-PCR. (A) Scheme showing the NCgl2816-lldD locus in C. glutamicum and the RT-PCRs used to determine cotranscription of NCgl2816 and lldD. RNA from wild-type C. glutamicum was transcribed into cDNA with two different primers in the two separate reverse transcriptase reactions cDNA-A and cDNA-B. Subsequently, these cDNAs were used as templates for the PCRs labeled 1 to 6. (B) Results from the RT-PCR analyses described above. The lower DNA fragment visible in lanes 1 to 6 represents dnaE, and RT-PCR of dnaE served as a positive control in all reactions. The upper bands correspond to the products of PCRs 1 to 6, diagramed in panel A. Lanes 7 to 12 represent control reactions confirming the absence of DNA in the RNA preparation. The reactions were identical to PCRs 1 to 6 (for which results are shown in lanes 1 to 6, respectively) except that reverse transcriptase was omitted in reactions cDNA-A and cDNA-B.