TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| C. glutamicum | ||
| ATCC 13032 | Biotin-auxotrophic wild type | ATCC |
| 13032::pK18mobNCgl2816int | NCgl2816 inactivation mutant of ATCC 13032 | This work |
| 2262 | Glutamate-producing strain | 41 |
| 2262NP | Non-glutamate-producing mutant of 2262 | 41 |
| E. coli DH5α | supE44 ΔlacU169 (φ80dlacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Invitrogen |
| Plasmids | ||
| pK18mob | Kanr; vector for integration into C. glutamicum; (pK18 oriVEc lacZα) | 35 |
| pK18mobNCgl2816int | pK18mob carrying an internal fragment of NCgl2816 | This work |
| pEKEx3 | Specr; C. glutamicum/E. coli shuttle vector for regulated gene expression (Ptac lacq pBL1 oriVCg pUC18 oriVEc) | E. Radmacher and L. Eggeling |
| pEKEx3-lldD | pEKEx3 carrying the NCgl2816/lldD gene | This work |