TABLE 3.

Comparative gene expression analysis of C. glutamicum ATCC 13032 grown on pyruvate or l-lactate as the sole carbon source

Genea	Annotationa	Relative mRNA levelb
NCgl0009	Putative transcriptional regulator	5.9
NCgl0014	Hypothetical membrane protein	4.1
NCgl0062	Putative chloride channel protein	4.0
NCgl0122	Hypothetical protein	10.2
NCgl0123	Hypothetical protein	7.6
NCgl0359	Succinate dehydrogenase, membrane anchor (sdhC)	0.2
NCgl0360	Succinate dehydrogenase, flavoprotein (sdhA)	0.2
NCgl0377	Putative heme transport-associated membrane protein	5.2
NCgl0430	ArsR-type regulator	4.2
NCgl0482	Putative iron siderophore ABC uptake system, ATPase	4.2
NCgl0943	Transcriptional regulator, AraC family	10.1
NCgl1170	Putative methylmalonyl coenzyme A epimerase	0.2
NCgl1262	3-Isopropylmalate dehydratase, large subunit (leuC)	0.2
NCgl1263	3-Isopropylmalate dehydratase, small subunit (leuD)	0.2
NCgl1470	Putative protein kinase	0.2
NCgl1472	Putative methylmalonyl coenzyme A mutase, beta subunit	0.2
NCgl1502	Predicted iron-regulated ABC-type transporter SufB	4.0
NCgl1646	Putative secreted hydrolase	7.0
NCgl1647	Putative secreted protein	5.0
NCgl2248	Isocitrate lysase (aceA)	0.2
NCgl2319	Catechol 1,2-dioxygenase	0.2
NCgl2353	Dipeptide-binding protein	4.1
NCgl2450	Hypothetical protein	6.7
NCgl2451	Hypothetical protein	4.5
NCgl2657	Phosphotransacetylase (pta)	0.2
NCgl2713	Putative transport protein	5.4
NCgl2719	Putative ferredoxin/ferredoxin-NADP reductase	4.2
NCgl2816	Putative transport protein	17.9
NCgl2817	Quinone-dependent l-lactate dehydrogenase (lldD)	17.4
NCgl2965	Putative transport protein	4.6

^aGene numbers and annotations of the revised C. glutamicum genome published by NCBI as NC_003450.

^bRatio of the mRNA level in l-lactate-grown cells to that in pyruvate-grown cells. Statistically significant changes of at least fourfold in gene expression determined in three independent experiments from independent cultivations (P < 0.05 by Student's t test) are listed.