TABLE 3.
P-β-galactosidase activity in L. lactis IL1403 and its ccpA and bglS mutants grown in CDM supplemented with various sugars
| Sugara | Enzymatic activity (nmol/min/mg of protein)b
|
|||
|---|---|---|---|---|
| IL1403 (wild-type) | IBB550 (ccpA) | bglS | bglS ccpA | |
| Glucose | 10.1 ± 3.35 | 28.98 ± 1.14 | 3.5 ± 0.25 | 12 ± 1.02 |
| Cellobiose-lactose | 123.73 ± 15.69 | 180.02 ± 11.39 | — | — |
| Lactose | —c | 276 ± 26.7 | — | — |
| Cellobiose | 192.11 ± 19.16 | 243.64 ± 32.2 | 24.44 ± 3.14 | 26.32 ± 1.64 |
| Galactose | 19.95 ± 2.53 | NDd | 14.47 ± 0.98 | ND |
| Galactose-cellobiose-lactose | 51.06 ± 2.35 | ND | 10.05 ± 2.54 | ND |
a
All sugars were used at the concentration of 1%; however, in media containing multiple sugars, the cellobiose concentration was 0.01%.
b
Mean values and standard deviations from at least three independent experiments are given.
c
—, unable to grow.
d
ND, not determined.