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. 2005 Oct;71(10):5685–5691. doi: 10.1128/AEM.71.10.5685-5691.2005

TABLE 2.

PCR primers and annealing temperatures used in this study

Primer Gene Sequence (5′ to 3′) Tmaa (°C)
LBT TTTTTACACTGATGAATGTTCCGTT 55
agfBF RT agfB TCCTGGTCTTCAGTAGCGTAA 54.7
agfBR RT TATGATGGAAGCGGATAAGAA
agfDF RT agfD TCCTGGTCTTCAGTAGCGTAA 59.1
agfDR RT TATGATGGAAGCGGATAAGAA
rplUF RT rplU CTGTTGAGTTCGCTGAAGTGC 57.2
rplUR RT CAGCATTTGTGATAGCATTCGTCG
a

Tma, annealing temperature used in PCR.