TABLE 2.
Effect of sulfide on the growth and survival of M. jannaschii and A. profundus at optimal (82°C) and superoptimal (88°C) temperatures with and without nutrients
| Sulfide concn (mM) for indicated species | Growth with growth nutrientsa
|
Survival without growth nutrientsb
|
||
|---|---|---|---|---|
| 82°C | 88°C | 82°C | 88°C | |
| M. jannaschii | ||||
| 0.4 | ++ | ++ | 4.5d | 3d |
| 10 | NDe | ND | 5d | 3d |
| 20 | ++ | ++ | 2 | 0 |
| 30 | ND | ND | 1 | 0 |
| 40 | ++ | ++ | 1 | 0 |
| 60 | ++ | − | ND | ND |
| 75/80c | + | − | 0 | 0 |
| A. profundus | ||||
| 0.4 | ++ | ++ | 4.5d | 2.5d |
| 10 | ND | ND | 2 | 2 |
| 20 | ++ | ++ | 1 | 0 |
| 30 | ND | ND | 0 | 0 |
| 40 | ++ | − | 0 | 0 |
| 60 | + | − | ND | ND |
| 75/80c | − | − | ND | ND |
a
Growth is indicated by an increase in cell number of greater than or equal to 1 order of magnitude (++), less than 1 order of magnitude (+), or less than one doubling (−).
b
Survival is indicated by the number of dilution steps in replicates of the sixfold dilution series that exhibited regrowth after 24-h exposure to nongrowth conditions.
c
Concentrations were 80 mM for the growth experiments and 75 mM for the survival experiments.
d
These numbers are the average of two experiments.
e
ND, no data collected.