TABLE 2.
Distributions of abundant RSTs associated with all samples (RST code C), boreal forest soils (RST code B), or arctic tundra soils (RST code A), corresponding taxonomic affiliations, and similarity score (S_ab) of the closest match in the RDP-II
| RST (DGGE band) | S_ab | Closest RDP affiliation | No. of RSTs ata:
|
RST Sequence | |||||
|---|---|---|---|---|---|---|---|---|---|
| CD | AL | NL | MM | NH | PR | ||||
| C1 (band C) | 1 | Unclassified Proteobacteria | 6 | 13 | 123 | 200 | 175 | 210 | AGCGGTAACAGGTGTAGCAATACATGCTGACGAGC |
| C2 (bands B and D) | 1 | Alpha Proteobacteria | 23 | 5 | 48 | 57 | 157 | 98 | AGCGGGCGTAGCAATACGTCAGC |
| C3 | 1 | Beta Proteobacteria | 16 | 36 | 10 | 39 | 72 | 15 | AACGGCAGCACGGGGGCAACCCTGGTGGCGAGT |
| C4 | 1 | Actinobacteria | 10 | 22 | 37 | 36 | 11 | 16 | AGCGGAAAGGCCCTTCGGGGTACTCGAGC |
| C5 | 1 | Beta Proteobacteria | 43 | 25 | 18 | 4 | 12 | 6 | AGCGGCAGCGCGGGGCAACCTGGCGGCGAGC |
| C6 | 1 | Beta Proteobacteria | 64 | 12 | 7 | 4 | 15 | 1 | AACGGCAGCACGGGAGCAATCCTGGTGGCGAGT |
| C7 | 1 | Gamma Proteobacteria | 5 | 1 | 66 | 23 | 4 | 2 | AGCGGTAACGCGGGAGCAATCCTGGCGACGAGC |
| C8 | 1 | Gamma Proteobacteria | 8 | 12 | 19 | 17 | 11 | 9 | AGCGGCAGCGCGGGGGCAACCCTGGCGGCGAGC |
| C9 | 1 | Unclassified | 4 | 2 | 15 | 28 | 5 | 1 | AACGCGAAAGTCCCGCAAGGGATCAGTAGAGT |
| C10 | 1 | Unclassified | 6 | 11 | 5 | 21 | 4 | 3 | AACGCGAAAGTCCCGCAAGGGATGAGTAGAGT |
| C11 | 1 | Acidobacteria | 1 | 5 | 9 | 24 | 4 | 2 | AACGAGAAAGGGGAGCAATCCCTGAGTAAAGT |
| C12 | 1 | Unclassified | 11 | 3 | 14 | 6 | 8 | 3 | AACGCGAAAGTCCCGCAAGGGATAAGTAGAGT |
| C13 | 1 | Alpha Proteobacteria | 2 | 2 | 7 | 2 | 20 | 8 | AACGCGTGTAGCAATACACGAGT |
| C14 | 1 | Gamma Proteobacteria | 3 | 1 | 5 | 8 | 9 | 13 | AACGGTAACAGGCCCGCAAGGGTGCTGACGAGT |
| C15 | 1 | Beta Proteobacteria | 2 | 7 | 5 | 3 | 13 | 2 | AACGGTAACGCGGGGGCAACCCTGGCGACGAGT |
| C16 | 1 | Unclassified Proteobacteria | 6 | 1 | 5 | 6 | 10 | 4 | AACGCGAAAGTCCCGCAAGGGATTAGTAGAGT |
| C17 | 1 | Alpha Proteobacteria | 6 | 1 | 11 | 3 | 3 | 3 | AACGATAAGCCACCTTCGGGTGGTGGACAGT |
| C18 | 1 | Alpha Proteobacteria | 1 | 4 | 9 | 3 | 3 | 1 | AACGCCCCGCAAGGGGAGT |
| B1 | 0.89 | Gamma Proteobacteria | 49 | AACGTGAAAGCGGGGCAACCCGCAAGTAGAGT | |||||
| B2 | 0.67 | Delta Proteobacteria | 33 | AGGGAGAAAGCCCGCAAGGGTTAGTAAACC | |||||
| B3 | 0.50 | Firmicutes | 24 | AACGAGCTGGTTTTCTTCGGAGAGCGAGCGAGT | |||||
| B4 | 0.50 | Alpha Proteobacteria | 16 | AACGCCCTTTGTCTCGCAAGAGAGGGAGGGAGT | |||||
| B5 | 1 | Unclassified | 12 | 1 | 1 | AACGCGCAGCAGTTTGTAGCAATACAGATTGTGGGCGCGT | |||
| B6 | 0.56 | Delta Proteobacteria | 14 | AACGCCCTTTGTCTCGCAAGAGAGGAAGGGAGT | |||||
| B7 | 0.75 | Aquificae | 14 | AACGGGTGCTAACTGCCCGCAAGGGT | |||||
| B8 | 0.68 | Unclassified | 13 | AACGCGCAGTTTCCTGTAGCAATACAGGGAATGGGCGCGT | |||||
| B9 | 0.60 | Actinobacteria | 12 | AACGGTGACCTCACTTCGGTGGGTGATCAGT | |||||
| B10 | 0.49 | Unclassified | 11 | AACGAGGATCATCGGGTTAGCAATAATTCGGTGGTCCTAGT | |||||
| B11 | 0.72 | Firmicutes | 5 | 6 | AGCGAATCTTTAGGAGCTTGCTCCTATTGGTTAGC | ||||
| B12 | 1 | Alpha Proteobacteria | 2 | 5 | 4 | AACGCGACCTTCGGGTCGAGT | |||
| B13 | 0.40 | Actinobacteria | 10 | AGCGTGGGCGTGCTGTCTCGCAAGAGATGGCACGTTCTAGC | |||||
| B14 | 1 | Unclassified | 10 | CACGAGAAACTCTGTAGCAATACGGGGCGGTAAAGT | |||||
| B15 | 0.52 | Firmicutes | 10 | AACGAGTTTGTCTTCTTCGGAAGATGAGCGAGT | |||||
| B16 | 1 | Nitrospira | 10 | AACGAGAAGGCGTAGCAATACGCTTGTAAAGT | |||||
| B17 | 0.96 | Delta Proteobacteria | 10 | AGCGAGAAAGGGGCAACCCCGGTAAAGC | |||||
| A1 (band A) | 1 | Gamma Proteobacteria | 284 | 23 | 42 | AACGGCAGCACAGAGGAGCTTGCTCCTTGGGTGGCGAGT | |||
| A2 | 1 | Beta Proteobacteria | 51 | 2 | AACGGTAGAGTAGCAATACTCGAGAGT | ||||
| A3 | 1 | Actinobacteria | 10 | 13 | AACGGTGAACCGGGCTTCGGCCCGGGGATCAGT | ||||
| A4 | 1 | Beta Proteobacteria | 10 | 10 | AACGGTAACGCGGGGCAACCTGGCGACGAGT | ||||
| A5 | 0.57 | Beta Proteobacteria | 19 | AACGGCAGCACGGGACTCAGGCAACTGAGCCCTGGTGGCGAGT | |||||
| A6 | 0.67 | Planctomycetes | 18 | AGCGAGAACCTAGGCTTCGGCTTAGGGGACAGC | |||||
| A7 | 0.78 | Gamma Proteobacteria | 17 | AACGGTAACAGACCCTTCGGGGTGCTGACGAGT | |||||
| A8 | 1 | Unclassified | 6 | 11 | AACGCGAAAGGGGCTTCGGCCCTGAGTAGAGT | ||||
| A9 | 0.46 | Cyanobacteria | 16 | TACGGGAAATATCCTAGTGGTGTTTCCAGT | |||||
| A10 | 1 | Delta Proteobacteria | 16 | AACGCGAAAGTCCTTCGGGATGAGTAAAGT | |||||
| A11 | 1 | Verrucomicrobia | 6 | 9 | AACGGGATTACTTTTGGTAGCAATACCGAAAGTGATTCAGT | ||||
| A12 | 1 | Actinobacteria | 11 | 3 | AGCGAGAACCAGGCCTTCGGGCCTGGGGACAGC | ||||
| A13 | 0.65 | Unclassified | 3 | 11 | AACGGGTGTTAGCTGCCCGCAAGGGT | ||||
| A14 | 0.89 | Gamma Proteobacteria | 12 | AACGGTAACGGGCCCTTCGGGGTGCTGACGAGT | |||||
| A15 | 0.49 | Verrucomicrobia | 11 | AACGGGAACTCTTTTGGTAGCAATACCGGGAGAGTTCTAGT | |||||
| A16 | 1 | Cyanobacteria | 11 | AACGGTAACAGGAAACAGCTTGCTGTTTCGCTGACGAGT | |||||
| A17 | 1 | Unclassified | 2 | 9 | AACGCGAAAGTCCCCTTCGGGGGGCGAGTAGAGT | ||||
| A18 | 1 | Actinobacteria | 11 | AGCGAGAACCGGACCTTCGGGTCCGGGGACAGC | |||||
| A19 | 0.59 | Alpha Proteobacteria | 11 | AACGGGGTCTTTCGGGATCTAGT | |||||
| A20 | 1 | Gamma Proteobacteria | 6 | 4 | AACGGCAGCACAGAGGAGCTTGCTCCTTGGGTGGCGAG | ||||
| A21 | 1 | Beta Proteobacteria | 10 | AGCGGACAGATGGGAGCTTGCTCCCTGATGTTAGC | |||||
| A22 | 0.89 | Unclassified | 9 | 1 | AACGCGAAAACCCCGCAAGGGGTTAGTAGAGT | ||||
| A23 | 0.64 | Firmicutes | 10 | AACGGATCTTTTCCTTCGGGGAAAGGTTAGT | |||||
| A24 | 1 | Actinobacteria | 10 | AACGAGAAAGCCCTTCGGGGTTAGTAAAGT | |||||
| A25 | 1 | Cyanobacteria | 10 | AACGGAATCTTTCGGGATTTAGT | |||||
a
CD, Cape Dyer; AL, Alert; NL, Nadluardjuk Lake; MM, Montmorency; NH, Narrow Hills; PR, Peace River.