TABLE 1.
Characterization by PFGE and PCR-RFLP of the different loci of the strains with different origins belonging to the laboratory collection
| Species | Strain | Animal | Year | Source | Geographic origin | PFGE profile
|
PCR-RFLP profile
|
Cluster-genotypeb | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| SmaI | KpnI | hipO | flaA | pflA/gyrA or rRNA genea | |||||||
| C. jejuni | CWH260199 | Human | 1999 | Hospital | Western France | S78 | K78 | h25 | fl10 | m5 | J35 |
| BOF | Human | 1994 | Hospital | Western France | NDc | K79 | h6 | fl6 | m8 | J36 | |
| UA580 | Human | 1983 | D. E. Taylor | Canada | S79 | K80 | h12 | fl9 | m12 | J37 | |
| ATCC 33560 | Bovine | 1991 | Pasteur Institute | France | S80 | ND | h26 | fl22 | m18 | J38 | |
| 3J01 | Poultry | 1994 | AFSSAd | Brittany, France | S81 | K81 | h6 | fl9A | m15 | J39 | |
| 3J4.5 | Poultry | 1994 | AFSSA | Brittany, France | S82 | K82 | h19 | fl17 | m13 | 15-J40 | |
| 3J32.30 | Poultry | 1994 | AFSSA | Brittany, France | S83 | K83 | h22 | fl1 | m6 | J41 | |
| A800 | Poultry | 1996 | Aerial | Eastern France | S84 | K84 | h1 | fl1 | m1 | 1-J42 | |
| A805 | Poultry | 1996 | Aerial | Eastern France | S7 | K5 | h1 | fl1 | m1 | 1-J1 | |
| A728 | Poultry | 1996 | Aerial | Eastern France | S85 | K85 | h20 | fl18 | m3 | 5-J43 | |
| A922 | Poultry | 1996 | Aerial | Eastern France | S86 | K86 | h19 | fl17 | m3 | 15-J44 | |
| A940 | Poultry | 1996 | Aerial | Eastern France | S87 | K87 | h21 | fl10 | m3 | J45 | |
| A943 | Poultry | 1996 | Aerial | Eastern France | S88 | K88 | h2 | fl21 | m17 | J46 | |
| A1020 | Poultry | 1996 | Aerial | Eastern France | S89 | K89 | h24 | fl20 | m16 | J47 | |
| A1306 | Poultry | 1996 | Aerial | Eastern France | S90 | K90 | h23 | fl19 | m4 | J48 | |
| C. coli | CWH020399 | Human | 1999 | Hospital | Western France | S62 | K60 | fl31 | rc7 | C35 | |
| UA417 | Human | 1983 | D. E. Taylor | Canada | S63 | K61 | fl23 | rc4 | C36 | ||
| A9821 | Pork | 1996 | Aerial | Eastern France | S64 | K62 | fl30 | rc9 | 14-C37 | ||
| A992 | Pork | 1996 | Aerial | Eastern France | S64 | K63 | fl30 | rc9 | 14-C38 | ||
| A1538 | Pork | 1996 | Aerial | Eastern France | S65 | K64 | fl30 | rc9 | C39 | ||
| A1581 | Pork | 1996 | Aerial | Eastern France | S66 | K65 | fl9A | rc2 | C40 | ||
| A1642 | Pork | 1996 | Aerial | Eastern France | S67 | K66 | fl9A | rc2 | C41 | ||
| A1635 | Pork | 1996 | Aerial | Eastern France | S68 | K67 | fl9A | rc2 | C42 | ||
| A1649 | Pork | 1996 | Aerial | Eastern France | S69 | K68 | fl10 | rc2 | 15-C43 | ||
| A1578 | Pork | 1996 | Aerial | Eastern France | S70 | K69 | fl10 | rc2 | 15-C44 | ||
| A1575 | Pork | 1996 | Aerial | Eastern France | S71 | K70 | fl32 | rc2 | 15-C45 | ||
| A1552 | Pork | 1996 | Aerial | Eastern France | S70 | K71 | fl33 | rc2 | 15-C46 | ||
| ATCC 33559 | Pork | 1980 | Pasteur Institute | France | S72 | K72 | fl9A | rc8 | C47 | ||
| MJ4.3 | Poultry | 1994 | AFSSA | Brittany, France | S73 | K73 | NCe | NC | C48 | ||
| A849 | Poultry | 1996 | Aerial | Eastern France | S74 | K74 | fl25 | rc2 | C49 | ||
| A879 | Poultry | 1996 | Aerial | Eastern France | S75 | K75 | fl4 | rc2 | 16-C50 | ||
| A846 | Poultry | 1996 | Aerial | Eastern France | S76 | K76 | fl4 | rc2 | 16-C51 | ||
| A963 | Poultry | 1996 | Aerial | Eastern France | S77 | K77 | Fl34 | rc10 | C52 | ||
a
pflA/gyrA PCR-RFLP profile for C. jejuni strains and rRNA gene profile for C. coli strains.
b
The number before the hyphen indicates the cluster to which the genotype belongs (e.g., in 15-J40 15 indicates cluster 15-J and in 14-C37 14 indicates cluster 14-C), and the number after the hyphen indicates the genotype. When there is no hyphen, the number indicates the genotype, which may belong to any cluster.
c
ND, not digested. The isolate was refractory to digestion by the restriction enzyme.
d
AFSSA, Agence Française de Sécurité Sanitaire des Aliments.
e
NC, isolate was not characterized by the typing method used.