TABLE 3.
Characterization by PFGE and PCR-RFLP of different loci of the C. jejuni isolates collected on the poultry farms
| Farm | Year | Total no. of typed isolates | PFGE
|
PCR-RFLP
|
Cluster-genotypea | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| SmaI | KpnI | No. of isolates | hipO | flaA | pflA/gyrA | No. of isolates | ||||
| A | 1996 | 67 | S7 | K5 | 17 | h1 | fl1 | m1 | 14 | 1-J1 |
| S8 | K6 | 13 | h2 | fl2 | m2 | 11 | J2 | |||
| S9 | K7 | 31 | h3 | fl3 | m3 | 20 | J3 | |||
| S7 | K8 | 4 | h1 | fl1 | m1 | 2 | 1-J4 | |||
| S10 | K9 | 2 | h4 | fl4 | m4 | 2 | J5 | |||
| B | 1997 | 358 | S19 | K19 | 174 | h4 | fl4 | m4 | 50 | 2-J6 |
| S19 | K20 | 26 | h4 | fl4 | m4 | 3 | 2-J7 | |||
| NDb | K21 | 7 | h6 | fl6 | m8 | 6 | 3-J8 | |||
| h6 | fl4 | m8 | 1 | 3-J8A | ||||||
| S20 | K22 | 101 | h5 | fl7 | m5 | 48 | 4-J9 | |||
| h5 | fl8 | m5 | 14 | 4-J9A | ||||||
| h5 | fl6 | m5 | 1 | 4-J9B | ||||||
| S21 | K23 | 1 | h1 | fl10 | m7 | 1 | 5-J10 | |||
| S22 | K24 | 12 | h9 | fl11 | m3 | 3 | J11 | |||
| S23 | K25 | 21 | h7 | fl9 | m9 | 16 | 6-J12 | |||
| h7 | fl7 | m9 | 1 | 6-J12A | ||||||
| S24 | K26 | 5 | h1 | fl1 | m6 | 5 | 7-J13 | |||
| S24 | K27 | 1 | h1 | fl1 | m6 | 1 | 7-J14 | |||
| S25 | K28 | 6 | h8 | fl1 | m10 | 3 | 8-J15 | |||
| h8 | fl9 | m10 | 2 | 8-J15A | ||||||
| S26 | ND | 3 | h9 | fl10 | m11 | 2 | 9-J16 | |||
| S27 | ND | 1 | h8 | fl1 | m10 | 1 | 9-J17 | |||
| C | 1998 | 225 | S33 | K33 | 1 | h10 | fl12 | m13 | 1 | J18 |
| S34 | K34 | 154 | h11 | fl9 | m10 | 73 | 10-J19 | |||
| h12 | fl9 | m12 | 4 | 10-J19A | ||||||
| S35 | K35 | 26 | h12 | fl9A | m12 | 9 | 11-J20 | |||
| h11 | fl9 | m10 | 5 | 11-J20A | ||||||
| h13 | fl7 | m7 | 2 | 11-J20B | ||||||
| S36 | K36 | 23 | h13 | fl7 | m7 | 20 | 12-J21 | |||
| h11 | fl9 | m10 | 1 | 12-J21A | ||||||
| S37 | K37 | 8 | h14 | fl9A | m6 | 8 | 13-J22 | |||
| S37 | K38 | 3 | h14 | fl9A | m6 | 3 | 13-J23 | |||
| S37 | K39 | 7 | h14 | fl9A | m6 | 5 | 13-J24 | |||
| h12 | fl9A | m10 | 1 | 13-J24A | ||||||
| h13 | fl7 | m7 | 1 | 13-J24B | ||||||
| S37 | K40 | 2 | h14 | fl9A | m6 | 2 | 13-J25 | |||
| S9 | K7 | 1 | h3 | fl3 | m3 | 1 | J3 | |||
| D | 1998 | 19 | S53 | K51 | 6 | h15 | fl13 | m4 | 1 | J26 |
| S54 | K52 | 5 | h4 | fl4 | m1 | 2 | J27 | |||
| S55 | K53 | 1 | h1 | fl1 | m1 | 1 | 1-J28 | |||
| S56 | K54 | 4 | h16 | fl14 | m14 | 2 | J29 | |||
| S57 | K55 | 1 | h6 | fl7 | m13 | 1 | 14-J30 | |||
| S58 | K56 | 2 | h17 | fl15 | m3 | 2 | J31 | |||
| F | 1999 | 11 | S59 | K57 | 8 | h18 | fl9A | m1 | 3 | J32 |
| S60 | K58 | 3 | h6 | fl16 | m13 | 3 | 14-J33 | |||
| G | 1999 | S61 | K59 | 23 | h1 | fl10 | m3 | 3 | J34 | |
a
See Table 1, footnote b.
b
ND, not digested. The isolate was refractory to digestion by the restriction enzyme.