FIG. 5.

Activation of Trichoderma MAPK by plant root interaction. Mycelia interacting with cucumber seedlings were harvested after 48 h (+). Mycelia grown in SM with 0.05% glucose were used as control (−). Cell lysates of T. asperellum (T-203), of the T. virens wild type (T.v-WT), and of the tmkA mutant (ΔMAPK-7) were analyzed by Western blotting using antibodies that specifically recognize either the dually phosphorylated forms of ERK1/2 or total ERK2 polypeptide.