TABLE 2.
Oligonucleotide primers and LC probes used in this studya
| Target | Oligonucleotide | Sequence (5′-3′) | Location | Product size (bp) |
|---|---|---|---|---|
| M. avium subsp. paratuberculosis | MAPf57p1 | TTG GAC GAT CCG AAT ATG T | 126-144 | 254 |
| F57 sequence (accession no. | MAPf57p2 | AGT GGG AGG CGT ACC A | 365-380 | |
| X70277) | MAPf57-3iFluo | CAC GCA GGC ATT CCA AGT | 250-267 | |
| MAPf57-5iRed705 | TGA CCA CCC TTC CCG TCG | 270-287 | ||
| pUC19 plasmid DNA (accession | PuC19fw | CGG AGA CGG TCA CAG CT | 49-65 | 400 |
| no. L09137) | PuC19rv | TTG CAT GCC TGC AGG T | 433-448 | |
| PuC19-3iFluo | GCA AGG CGA TTA AGT TGG GTA AC | 330-350 | ||
| PuC19-5iRed640 | CAG GGT TTT CCC AGT CAC GAC | 355-375 |
a
All oligonucleotides were synthesized by Microsynth (Balgach, Switzerland), and all probes were selected using the LC Probe Design software and were synthesized by TIB-Molbiol (Berlin, Germany).