Table 1.
Properties and sequence similarities of the C. glutamicum proteins possibly involved in assimilatory reduction of sulphate
| C. glutamicum | protein properties | BLAST resultsb | ||||||
| CDS (protein a ) | length (aa) | pI | mw [kDa] | hit against | in organismc | description | E-value | identical/positive aa |
| Cg3112 (CysZ) | 309 | 9.98 | 32.0 | Q8FM69 | C. efficiens | Conserved hypothetical protein | 2e-134 | 77/88% |
| Q81NU8 | B. anthracis | Putative membrane protein | 8e-58 | 46/64% | ||||
| Q813F4 | B. cereus | Hypothetical membrane spanning protein | 4e-57 | 46/64% | ||||
| Cg3113 (CysY) | 241 | 5.44 | 24.7 | Q8FM68 | C. efficiens | Hypothetical protein | 1e-80 | 67/77% |
| P61817 | B. megaterium | Sirohydrochlorin ferrochelatase SirB | 5e-16 | 28/45% | ||||
| Q93RW8 | S. coelicolor | Hypothetical protein SC01858 | 2e-13 | 31/43% | ||||
| Cg3114 (CysN) | 433 | 5.08 | 46.9 | Q8FM67 | C. efficiens | Putative sulphate adenylyltransferase SU 1 | 0.0 | 84/90% |
| Q9ADG6 | S. coelicolor | Sulphate adenylyltransferase SU 1 | 3e-lll | 55/67% | ||||
| Q10600 | M. tuberculosis | Sulphate adenylyltransferase SU 1 | 1e-104 | 50/69% | ||||
| Cg3115 (CysD) | 304 | 5.09 | 34.3 | Q8FM66 | C. efficiens | Putative sulphate adenylyltransferase | 7e-168 | 95/96% |
| Q9ADG5 | S. coelicolor | Sulphate adenylyltransferase SU 2 | 2e-123 | 71/83% | ||||
| Q9X5UO | S. lavendulae | Sulphate adenylyltransferase SU 2 | 6e-123 | 70/82% | ||||
| Cg3116 (CysH) | 261 | 5.30 | 28.5 | Q8FM65 | C. efficiens | Putative PAPS reductase | 3e-129 | 86/89% |
| Q82L82 | S. avermitilis | Putative PAPS reductase | 7e-52 | 56/67% | ||||
| P71752 | M. tuberculosis | APS reductase | 5e-52 | 54/69% | ||||
| Cg3117 (CysX) | 82 | 7.86 | 9.5 | Q8FM64 | C. efficiens | Hypothetical protein | 5e-36 | 81/89% |
| Q82L83 | S. avermitilis | Hypothetical protein | 4e-05 | 50/57% | ||||
| Q9ADG2 | S. coelicolor | Hypothetical protein SC06101 | 5e-05 | 50/57% | ||||
| Cg3118 (CysI) | 561 | 5.53 | 63.0 | Q8FM63 | C. efficiens | Putative ferredoxin-nitrite reductase | 0.0 | 91/95% |
| Q7WT38 | Streptomyces sp. | Nitrile/sulphite reductase | 0.0 | 56/70% | ||||
| Q82L84 | S. avermitilis | Putative nitrile/sulphite reductase | 0.0 | 55/71% | ||||
| Cg3119 (Fpr2) | 457 | 4.88 | 50.1 | Q8FM62 | C. efficiens | Putative ferredoxin-NADP reductase | 0.0 | 87/93% |
| Q8NM28 | C. glutamicum | NADPH-glutamate synthase beta chain | 0.0 | 74/86% | ||||
| Q8FMB5 | C. efficiens | Putative ferredoxin/adrenodoxin reductase | 0.0 | 75/85% | ||||
| FPRA_MYCTU | M. tuberculosis | Ferredoxin-NADP reductase FprA | 6e-83 | 39% | ||||
| FPRB_MYCTU | M. tuberculosis | Ferredoxin-NADP reductase FprB | 63e-66 | 36% | ||||
a by sequence similarity
b identified by BLASTP similarity search. The protein sequences derived from the C. glutamicum candidate CDS were used as queries against the UniProt database
c B. = Bacillus, C. = Corynebacterium, M. = Mycobacterium, S. = Streptomyces