Table 2.
Growth of C. glutamicum mutant strains on different inorganic sulphur sources on solid minimal medium
deleted | growtha after 48 h on MMS with addition of 2 mM of | |||||
strain | CDS (gene) | sulphate | sulphite | thiosulphate | sulphide | L-cysteine |
WT | / | + | + | + | + | + |
CR018 | cg3112 (cysZ ) | - | - | + | + | + |
CR019 | cg3113 (cysY ) | - | - | - | ° | + |
CR020 | cg3114 (cysN ) | - | - | ° | + | + |
CR021 | cg3115 (cysD ) | - | - | ° | + | + |
CR022 | cg3116 (cysH ) | - | - | ° | + | + |
CR023 | cg3117 (cysX ) | ° | ° | ° | + | + |
CR024 | cg3118 (cysI ) | - | - | - | ° | + |
CR025 | cg3119 (fpr2 ) | + | + | + | + | + |
a compared to the wild-type grown on MMS containing sulphate:
+ denotes wild-type like growth
° indicates a severely reduced growth rate
- represents a complete lack of growth