Table 1.
Relative effects of D184 substitutions on [PSI+] and Hsp104 activity
| Residue 184 | [PSI+] phenotype
|
Hsp104 activity
|
||||
|---|---|---|---|---|---|---|
| −ade growth
|
Stability
|
|||||
| G0 | G3 | G0 | G3 | G0 | G5 | |
| D (Asp) | 5 | 0 | 5 | 0 | 5 | 0 |
| S (Ser) | 5 | 4 | 5 | 5 | 5 | 4 |
| A (Ala) | 5 | 4 | 5 | 5 | ND | ND |
| Q (Gln) | 5 | 4 | 5 | 4 | 3 | 2 |
| L (Leu) | 5 | 4 | 5 | 3 | ND | ND |
| N (Asn) | 5 | 3 | 5 | 3 | 3 | 2 |
| F (Phe) | 5 | 3 | 5 | 2 | ND | ND |
| Y (Tyr) | 2 | 2 | 2 | 0 | 4 | 2 |
| W (Trp) | 0 | 0 | 0 | 0 | 2 | 0 |
| K (Lys) | 0 | 0 | 0 | 0 | 0 | 0 |
| Null | 0 | 0 | 0 | 0 | 0 | 0 |
G0, G3, and G5 indicate that assay conditions included 0, 3, or 5 mM guanidine, respectively. Scores range from that of wild type (D) without guanidine (5 = fastest −ade growth and completely stable) to that of the hsp104 null mutant (0 = Ade− and [psi−]). ND, not determined.