Fig. 1.

Time course of histone H3 assembly on HSV lytic-gene promoters during infection of murine trigeminal ganglion neurons. (A) Total HSV DNA levels in ganglia. At the times indicated, mice were euthanized, trigeminal ganglia were removed, lysates were prepared, and real-time PCR was performed. The values for HSV ICP4 gene sequences shown were determined for each time point and normalized to the amount in the ganglia at day 1 and then normalized to the levels of the cellular GAPDH gene, which was quantified in a separate PCR. (B) ChIP of viral DNA associated with histone H3. ChIP was conducted on the lysates in A by using anti-histone H3 antibody as the primary antibody. Viral DNA sequences were quantified by using primers for the ICP4 or TK gene promoters for real-time PCR. The fraction of the viral DNAs immunoprecipitated was normalized to the fraction of GAPDH DNA immunoprecipitated in the same immunoprecipitate to give the fold-enrichment values.