Table 1.
Summary of results using 28 variola genomes (finished or simulated draft as indicated) and 22 NN genomes from the Orthopox family, as well as the finished and draft Marburg results
| Species | Simulated draft or finished target | Draft or finished NNs | Percent conserved sequence (%) | Percent conserved and unique sequence (%) | Number TaqMan DNA signature candidates | Number conserved and unique regions | Longest conserved and unique region |
|---|---|---|---|---|---|---|---|
| Variola major virus | Simulated draft, high error rate | Finished | 58.30 | 57.79 | 0 | 4 | 23 |
| Variola major virus | Simulated draft, high error rate | Simulated draft, high error rate | 58.68 | 58.36 | 0 | 8 | 23 |
| Variola major virus | Finished | Simulated draft, high error rate | 98.90 | 3.91 | 1 | 71 | 49 |
| Variola major virus | Simulated draft, low error rate | Finished | 98.61 | 4.60 | 1 | 89 | 49 |
| Variola major virus | Simulated draft, low error rate | Simulated draft, low error rate | 98.65 | 4.43 | 0 | 86 | 49 |
| Variola major virus | Simulated draft, low error rate | Simulated draft, high error rate | 98.76 | 4.42 | 0 | 88 | 49 |
| Variola major virus | Simulated draft, intermediate error rate | Simulated draft, high error rate | 96.67 | 14.06 | 0 | 191 | 52 |
| Variola major virus | Finished | Simulated draft, low error rate | 98.84 | 4.05 | 1 | 80 | 49 |
| Variola major virus | Finished | Finished | 98.90 | 3.99 | 1 | 76 | 49 |
| Marburg virus | Real draft, 3× to 6× coverage | Finished | 92.60 | 83.31 | 43 | 250 | 198 |
| Marburg virus | Finished | Finished | 75.19 | 74.36 | 0 | 38 | 41 |
The percent of the target genome that is conserved varies slightly among the runs using finished target sequences because different genomes were randomly selected to be the reference strain in each multiple sequence alignment.