Table 3.

EF-G binding to the different ribosomal complexes, as monitored by protection from chemical modification

Ribosomal complex	WT	C2394G
Relative modification of the nucleotide 2661
Ribosomes, MFK-mRNA, fMet-${\text{tRNA}}_{\text{f}}^{\text{Met}}$, Phe-tRNAPhe*EF-Tu*GTP, EF-G*GTP, fusidic acid	0.79 ± 0.04	0.90 ± 0.05
Ribosomes, MFK-mRNA, fMet-${\text{tRNA}}_{\text{f}}^{\text{Met}}$, Phe-tRNAPhe*EF-Tu*GTP, EF-G*GMPPNP	0.31 ± 0.06	0.56 ± 0.05
Ribosomes, EF-G*GTP, fusidic acid	0.59 ± 0.10	0.73 ± 0.08
Ribosomes, EF-G*GMPPNP	0.11 ± 0.04	0.37 ± 0.04
Relative modification of the nucleotide 1067
Ribosomes, MFK-mRNA, fMet-${\text{tRNA}}_{\text{f}}^{\text{Met}}$, Phe-tRNAPhe*EF-Tu*GTP, EF-G*GTP, fusidic acid	0.86 ± 0.10	1.01 ± 0.10
Ribosomes, MFK-mRNA, fMet-${\text{tRNA}}_{\text{f}}^{\text{Met}}$, Phe-tRNAPhe*EF-Tu*GTP, EF-G*GMPPNP	0.62 ± 0.07	0.74 ± 0.05
Ribosomes, EF-G*GTP, fusidic acid	0.73 ± 0.10	0.80 ± 0.05
Ribosomes, EF-G*GMPPNP	0.50 ± 0.05	0.63 ± 0.04