Table 4.
Effect of the C2394G mutation on translational fidelity
| Test plasmid | Type of translation error | WT | C2394G |
|---|---|---|---|
| pSG 853 | UAA readthrough | 2.2 ± 0.6 | 7.3 ± 0.6 |
| pSG 12-6 | UAG readthrough | 7 ± 0.4 | 12 ± 0.5 |
| pSG 3/4 | UGA readthrough | 43 ± 2 | 64 ± 3 |
| pSG lac7 | +1 frameshift | 41 ± 2 | 74 ± 4 |
| pSG 12DP | −1 frameshift | 44 ± 2 | 84 ± 5 |
| f'CSH 103 | Glu/Gln substitution | 0.6 ± 0.1 | 0.6 ± 0.1 |
| pSG25 | no error | 4280 ± 70 | 5990 ± 80 |
Values are expressed in Miller units of the β-galactosidase activity. The higher is the activity, the more frequent is the translational error event.